Effect of Cinnamon Leaf Extract (Cinnamomum Burmannii) on Inhibiting Biofilm Formation in Klebsiella Pneumoniae in Vitro
Main Article Content
Klebsiella pneumoniae is an opportunistic pathogenic bacterium that can cause various nosocomial infections such as pneumonia, bacteremia, and urinary tract infections. These bacteria can form biofilms, which facilitate resistance to antibiotics. Cinnamon (Cinnamomum burmannii) is a native Indonesian spice that contains antibiofilm compounds such as flavonoids, tannins, and saponins. This study aimed to prove the effectiveness of Cinnamon leaves ethanol extract in inhibiting biofilm formation in Klebsiella pneumoniae in vitro. The method used was the tube method. The results from this method were documented and quantified as Mean Gray Value (MGV) using Adobe Photoshop CS6. The results showed that MGV increased with higher concentrations of Cinnamon leaves extract, indicating inhibition of biofilm formation in Klebsiella pneumoniae. The Minimum Biofilm Inhibitory Concentration (MBIC) was determined to be 37.5%. A one-way ANOVA test revealed a significant difference between the mean values of each group (p=0.000), and the Pearson correlation test showed a very strong and significant correlation (r=0.841; p=0.000). This study concludes that Cinnamon leaves ethanol extract can inhibit biofilm formation in Klebsiella pneumoniae in vitro. The implications of this research suggest that Cinnamon leaves ethanol extract could be explored as a potential natural alternative to conventional antibiotics in managing biofilm-related infections, particularly those caused by multidrug-resistant Klebsiella pneumoniae. Further studies are needed to evaluate the efficacy of this extract in vivo and its potential for clinical application.
Al-Abeadi, R. S., Suhail, A. A., & Almousawi, A. E. (2023). An overview of Klebsiella pneumoniae: History, taxonomy, epidemiology, pathogenicity, virulence factors and treatment. AIP Conference Proceedings, 2977(1).
Ali, A., Zahra, A., Kamthan, M., Husain, F. M., Albalawi, T., Zubair, M., Alatawy, R., Abid, M., & Noorani, M. S. (2023). Microbial biofilms: applications, clinical consequences, and alternative therapies. Microorganisms, 11(8), 1934.
Asfour, H. Z. (2018). Anti-quorum sensing natural compounds. Journal of Microscopy and Ultrastructure, 6(1), 1–10.
Aslan, S., Hasanuddin, N. R., Mallombasang, A. T. B., & AR, N. A. (2022). Effectiveness of Mixed Clove Flower Extract (Syzygium Aromaticum) And Sweet Wood (Cinnamon Burmanni) on the Growth of Enterococcus Faecalis. Indian Journal of Forensic Medicine & Toxicology, 16(1), 1089–1094.
Cui, H., Zhang, C., Li, C., & Lin, L. (2020). Inhibition mechanism of cardamom essential oil on methicillin-resistant Staphylococcus aureus biofilm. LWT, 122, 109057. https://doi.org/10.1016/j.lwt.2020.109057
Holban, A. M., Gestal, M. C., & Grumezescu, A. M. (2016). Control of biofilm-associated infections by signaling molecules and nanoparticles. International Journal of Pharmaceutics, 510(2), 409–418. https://doi.org/10.1016/j.ijpharm.2016.02.044
Liu, S., Yang, L., Zheng, S., Hou, A., Man, W., Zhang, J., Wang, S., Wang, X., Yu, H., & Jiang, H. (2021). A review: the botany, ethnopharmacology, phytochemistry, pharmacology of Cinnamomi cortex. RSC Advances, 11(44), 27461–27497.
Paterson, S. (2017). Biofilms: their importance in veterinary medicine. Companion Animal, 22(11), 659–668.
Sakulsathaporn, A., Chantasit, P., Kwantrairat, S., Jirukkakul, N., & Sripa, S. (2025). Red-Blue LED lights promoting on biomass production and MRSA inhibition properties in callus cultures of the endemic Thai plant Platostoma hemratianum Suddee, Puudjaa & Kiewbang (Lamiaceae). Plant Cell, Tissue and Organ Culture (PCTOC), 160(1), 13.
Santiago, C., Lim, K.-H., Loh, H.-S., & Ting, K. N. (2015). Inhibitory effect of Duabanga grandiflora on MRSA biofilm formation via prevention of cell-surface attachment and PBP2a production. Molecules, 20(3), 4473–4482.
Shariati, A., Didehdar, M., Razavi, S., Heidary, M., Soroush, F., & Chegini, Z. (2022). Natural compounds: a hopeful promise as an antibiofilm agent against Candida species. Frontiers in Pharmacology, 13, 917787.
Shariati, A., Noei, M., Askarinia, M., Khoshbayan, A., Farahani, A., & Chegini, Z. (2024). Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community. Frontiers in Pharmacology, 15, 1350391.
Sharma, S., Mohler, J., Mahajan, S. D., Schwartz, S. A., Bruggemann, L., & Aalinkeel, R. (2023). Microbial biofilm: a review on formation, infection, antibiotic resistance, control measures, and innovative treatment. Microorganisms, 11(6), 1614.
Shineh, G., Mobaraki, M., Perves Bappy, M. J., & Mills, D. K. (2023). Biofilm formation, and related impacts on healthcare, food processing and packaging, industrial manufacturing, marine industries, and sanitation–a review. Applied Microbiology, 3(3), 629–665.
Silva, N. L. F., Vieira, P. de B., Silva, M. V. da, Macedo, A. J., & Tasca, T. (2024). The Enhanced Activity of a Plant Mixture from the Brazilian Caatinga Biome against Venereal Trichomonads Confirms the Traditional Use. Venereology, 3(1), 15–25.
Slobodníková, L., Fialová, S., Rendeková, K., Ková?, J., & Mu?aji, P. (2016). Antibiofilm activity of plant polyphenols. Molecules, 21(12), 1717.
Susanti, E., Wahjuningrum, D., Nuryati, S., & Setiawati, M. (2021). The effectiveness of cinnamon powder and cinnamon leaf extract to prevent Aeromonas hydrophila infection on striped catfish Pangasianodon hypophthalamus. Jurnal Akuakultur Indonesia, 20(2), 163–173.
Tocci, N., Weil, T., Perenzoni, D., Narduzzi, L., Madriñán, S., Crockett, S., Nürk, N. M., Cavalieri, D., & Mattivi, F. (2018). Phenolic profile, chemical relationship and antifungal activity of Andean Hypericum species. Industrial Crops and Products, 112, 32–37. https://doi.org/10.1016/j.indcrop.2017.10.030
Varalakshmi, B., Firdous, J., Manikandan, R., Karpagam, T., Shanmugapriya, A., Abarna, V., & Jeyachristy, S. A. (2025). Antibiofilm Efficacy of Phenolic Rich Fraction from Cinnamomum zeylanicum Bark against Pseudomonas aeruginosa. International Journal of Advancement in Life Sciences Research, 8(1), 89–99.
Vl?ko, T., Rathod, N. B., Kulawik, P., Ozogul, Y., & Ozogul, F. (2022). The impact of aromatic plant-derived bioactive compounds on seafood quality and safety (pp. 275–339). https://doi.org/10.1016/bs.afnr.2022.05.002
Yadav, N., Saini, N., Rathi, N., Chauhan, S., Sharma, P. K., & Sangwan, N. S. (2025). Health-promoting effects of cinnamon. In Cinnamon (pp. 219–241). Elsevier. https://doi.org/10.1016/B978-0-443-21820-0.00015-5
