Ultrasound-Assisted Synthesis of Mangosteen Nanoparticles: Characterization of Particle Size, Phytochemical Content, Antibacterial, and Antioxidant Properties
Main Article Content
Diabetic foot wounds present a significant challenge in clinical management, necessitating innovative wound care approaches. This study evaluated the effectiveness of Garcinia mangostana Linn nanoparticle spray in promoting wound healing in diabetic foot wounds, focusing on wound size, TNF-? levels, and fibroblast activity. Using an experimental design with a diabetic animal model, the study assessed the impact of Garcinia mangostana Linn nanoparticle spray on these key parameters. Statistical analysis revealed a significant reduction in wound size (p=0.013), a notable decrease in TNF-? levels (p=0.001), and a strong fibroblast response (p=0.000), indicating enhanced collagen production and tissue formation. These findings suggest that Garcinia mangostana Linn nanoparticle spray has promising therapeutic potential in diabetic wound care by reducing inflammation and accelerating tissue regeneration.
Keywords:
Diabetic foot wound, Garcinia mangostana Linn, nanoparticle spray, TNF-?, fibroblasts, wound healing.
Zhang P, Lu J, Jing Y, Tang S, Zhu D, Bi Y. Global Epidemiology of Diabetic Foot Ulceration: A Systematic Review and Meta-Analysis. Ann Med [Internet]. 2016; (September). Available from: http://linkinghub.elsevier.com/retrieve/pii/S1877042815024659
IDF. IDF Diabetes Atlas. Eighth edition. Karuranga S, Fernandes J da R, Huang Y, Malanda B, editors. International Diabetes Federation; 2017. 40-63 p.
WHO Library Cataloguing-in-Publication Data. World Health Organization [Internet]. [cited 2018 May 31]; Available from: http://apps.who.int/iris/bitstream/handle/10665/97852/9789241596299_eng.pdf?sequence=1&isAllowed=y
Guo S, DiPietro LA. Critical review in oral biology & medicine: Factors affecting wound healing. J Dent Res. 2010;89(3):219-29.
Valenzuela-Silva CM, Tuero-Iglesias AD, Garcia-Iglesias E, Gonzalez-Diaz O, Del Rio-Martin A, Alos IBY, et al. Granulation response and partial wound closure predict healing in clinical trials on advanced diabetic foot ulcers treated with recombinant human epidermal growth factor. Diabetes Care. 2013;36(2):210-5.
Yip WL. Influence of oxygen on wound healing. 2015;
Zamboni WA, Browder LK. Hyperbaric oxygen and wound healing. 2012; (May).
Sgonc R, Gruber J. Age-Related Aspects of Cutaneous Wound Healing: A Mini-Review. 2013;159-64.
International consensus roundtable meeting identification and management of diabetic foot ulcers [Internet]. Wound International. 2017. Available from: http://www.nejm.org/doi/10.1056/NEJMra1615439
Rahimi S, Dombrovskiy VY, Ventarola D, Vatankhah N, Abraham C, Alkayed N, et al. Location of Diabetic Foot Ulcer Affects Wound Outcomes. 2017 [cited 2019 Jun 24]; Available from: https://www.jvascsurg.org/article/S0741-5214(17)30733-4/pdf
Lipsky BA, Aragón-Sánchez J, Diggle M, Embil J, Kono S, Lavery L, et al. IWGDF guidance on the diagnosis and management of foot infections in persons with diabetes. Diabetes Metab Res Rev [Internet]. 2016 Jan [cited 2019 Feb 1];32:45-74. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26386266
Sadriwala QS, Gedam BS, Akhtar MA. Risk factors of amputation in diabetic foot infections. Int Surg J. 2018;5(4):1399-402.
Pereira SG, Moura J, Carvalho E, Empadinhas N. Microbiota of chronic diabetic wounds: Ecology, impact, and potential for innovative treatment strategies. Front Microbiol. 2017;8(SEP):1-12.
Peters EJ, Lipsky BAJ, Aragón-Sánchez EJ, Boyko, Diggle M, J.M.Embil S, et al. Interventions in the management of infection in the foot in diabetes: a systematic review. Diabetes Metab Res Rev. 2016;32(1):145-53.
Lima AF, Costa LB, Silva JL Da, Maia MBS, Ximenes ECPA. Interventions for wound healing among diabetic patients infected with Staphylococcus aureus: a systematic review. Sao Paulo Med J. 2011;129(3):165-70.
Nube V, Frank G, White J, Stubbs S, Nannery S, Pfrunder L, et al. Hard-to-heal diabetes-related foot ulcers: current challenges and future prospects. 2016;133-46.
Anderson K, Hamm RL. Factors that impair wound healing. J Am Coll Clin Wound Spec [Internet]. 2012;4(4):84-91. Available from: http://dx.doi.org/10.1016/j.jccw.2014.03.001
Alexiadou K, Doupis J. Management of Diabetic Foot Ulcers. Diabetes Ther. 2012;3(4):1-15.
Rustenbach SJ, Augustin M, Herberger K, Spehr C, Heyer K, Protz K. Effectiveness of Advanced versus Conventional Wound Dressings on Healing of Chronic Wounds: Systematic Review and Meta-Analysis. Dermatology. 2013;226(2):172-84.
Tricco AC, Cogo E, Isaranuwatchai W, Khan PA, Sanmugalingham G, Antony J, et al. A systematic review of cost-effectiveness analyses of complex wound interventions reveals optimal treatments for specific wound types. BMC Med. 2015;13(1):1-16.
Kvitkina T, Narres M, Claessen H, Droste S, Morbach S, Kuss O, et al. Incidence of lower extremity amputation in the diabetic compared to the non-diabetic population: A systematic review protocol. Syst Rev [Internet]. 2015;4(1). Available from: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L605593026%5Cnhttp://dx.doi.org/10.1186/s13643-015-0064-9%5Cnhttp://sfx.library.uu.nl/utrecht?sid=EMBASE&issn=20464053&id=doi:10.1186%2Fs13643-015-0064-9&atitle=Incidence+of+lower+ex
Alqadri, Tambing Y, Latarang B. Morphological and anatomical characteristics of mangosteen (Garcinia mangostana L.). Agrotekbis. 2016;4(5):571-8.
Nainwal P, Bhagla.A, Nanda.D. Study on antioxidant potential and wound healing activity on the aqueous extract of fruits of Garcinia mangostana. IJPI's J Pharmacogn Herb Formul. 2014;1. Study on antioxidant potential and wound healing activity on the a. IJPI's J Pharmacogn Herb Formul. 2014;1.
Widowati W, Darsono L, Suherman J, Fauziah N, Maesaroh M, Erawijantari P putu. Anti-inflammatory effect of mangosteen (Garcinia mangostana l.) peel extract and its compounds in lps-induced raw264.7 cells. Nat Prod Sci. 2016;22(3):147-53.
Jindarat S. Xanthones from mangosteen (Garcinia mangostana): Multi-targeting pharmacological properties. J Med Assoc Thail. 2014;97(2):S196-201.
Taher M, Muhamad T, Syafiq F, Zakaria T, Susanti D, Zakaria ZA. Hypoglycaemic activity of ethanolic extract of Garcinia mangostana Linn. in normoglycaemic and streptozotocin- induced diabetic rats. BMC Complement Altern Med [Internet]. 2016;16(135):1-12. Available from: http://dx.doi.org/10.1186/s12906-016-1118-9
Nganlasom J, Suttitum T, Jirakulsomchok D, Puapairoj A. Effects of Centella Asiatica Linn. Leaves and Garcinia Mangostana Linn. hull on the Healing of Dermal Wounds in Diabetic Rats. Srinagarind Med J. 2008;23(4):402-7.
Cholilah, Ellistasari EY, Radiono S. Effects of 5%, 10%, 20% and 40% mangosteen (garcinia mangostana l.) peel extract cream on wound healing in vivo on mice skin [Internet]. Gadjah Mada University; 2017. Available from: http://etd.repository.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=109968&obyek_id=4
Aalaa M, Malazy OT, Sanjari M, Peimani M, Mohajeri-Tehrani MR. Nurses' role in diabetic foot prevention and care; a review. J Diabetes Metab Disord. 2012;11(1):1-6.
Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem [Internet]. 2017;34:540-60. Available from: http://dx.doi.org/10.1016/j.ultsonch.2016.06.035
Santos D, Vardanega R, De Almeida MA. Intensification of bioactive compounds extraction from medicinal plants using ultrasonic irradiation. Pharmacogn Rev [Internet]. 2014;8(16):88. Available from: http://www.phcogrev.com/text.asp?2014/8/16/88/134231
Alberti T, Coelho DS, Voytena A, Pitz H, Prá M De, Mazzarino L. Nanotechnology: A Promising Tool Towards Wound Healing. Pharmaceutical. 2017;23(January 2018):1.
Goyal R, Macri LK, Kaplan HM, Kohn J. Nanoparticles and nanofibers for topical drug delivery. J Control Release [Internet]. 2016 [cited 2019 Jun 27];240:77-92. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26518723
Singla R, Soni S, Patial V, Kulurkar PM, Kumari A, Mahesh S, et al. In vivo diabetic wound healing potential of nanobiocomposites containing bamboo cellulose nanocrystals impregnated with silver nanoparticles. Int J Biol Macromol [Internet]. 2017;105:45-55. Available from: http://dx.doi.org/10.1016/j.ijbiomac.2017.06.109
Patra JK, Das G, Fraceto LF, Vangelie E, Campos R, Rodriguez P, et al. Nano-based drug delivery systems: recent developments and future prospects. J Nanobiotechnology [Internet]. 2018;1-33. Available from: https://doi.org/10.1186/s12951-018-0392-8
Das S, Baker AB. Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing. Front Bioeng Biotechnol [Internet]. 2016;4(October):1–20. Available from: http://journal.frontiersin.org/article/10.3389/fbioe.2016.00082/full
Schneider M, Stracke F, Hansen S, Schaefer UF. Nanoparticles and their interactions with the dermal barrier. Dermatoendocrinol. 2010;1(4):197-206.
Grice JE, Zvyagin A V, Roberts M, Liu X. Penetration of Nanoparticles into Human Skin. Curr Pharm Des. 2013;19(March):6353.
Wani A, Sanghani C, Wani S. Formulation, Characterization, and in Vitro Evaluation of Novel Microemulsion-Based Spray for Topical Delivery of Isotretinoin. Asian J Pharm Clin Res. 2018;11(10):226.
Hasan AEZ, Nashrianto H, Juhaeni RN, Artika IM. Optimization of conditions for flavonoids extraction from Mangosteen (Garcinia mangostana L.). Der Pharm Lett. 2016;8(18):114-20.
IDF. IDF Diabetes Atlas. Eighth edition. Karuranga S, Fernandes J da R, Huang Y, Malanda B, editors. International Diabetes Federation; 2017. 40-63 p.
WHO Library Cataloguing-in-Publication Data. World Health Organization [Internet]. [cited 2018 May 31]; Available from: http://apps.who.int/iris/bitstream/handle/10665/97852/9789241596299_eng.pdf?sequence=1&isAllowed=y
Guo S, DiPietro LA. Critical review in oral biology & medicine: Factors affecting wound healing. J Dent Res. 2010;89(3):219-29.
Valenzuela-Silva CM, Tuero-Iglesias AD, Garcia-Iglesias E, Gonzalez-Diaz O, Del Rio-Martin A, Alos IBY, et al. Granulation response and partial wound closure predict healing in clinical trials on advanced diabetic foot ulcers treated with recombinant human epidermal growth factor. Diabetes Care. 2013;36(2):210-5.
Yip WL. Influence of oxygen on wound healing. 2015;
Zamboni WA, Browder LK. Hyperbaric oxygen and wound healing. 2012; (May).
Sgonc R, Gruber J. Age-Related Aspects of Cutaneous Wound Healing: A Mini-Review. 2013;159-64.
International consensus roundtable meeting identification and management of diabetic foot ulcers [Internet]. Wound International. 2017. Available from: http://www.nejm.org/doi/10.1056/NEJMra1615439
Rahimi S, Dombrovskiy VY, Ventarola D, Vatankhah N, Abraham C, Alkayed N, et al. Location of Diabetic Foot Ulcer Affects Wound Outcomes. 2017 [cited 2019 Jun 24]; Available from: https://www.jvascsurg.org/article/S0741-5214(17)30733-4/pdf
Lipsky BA, Aragón-Sánchez J, Diggle M, Embil J, Kono S, Lavery L, et al. IWGDF guidance on the diagnosis and management of foot infections in persons with diabetes. Diabetes Metab Res Rev [Internet]. 2016 Jan [cited 2019 Feb 1];32:45-74. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26386266
Sadriwala QS, Gedam BS, Akhtar MA. Risk factors of amputation in diabetic foot infections. Int Surg J. 2018;5(4):1399-402.
Pereira SG, Moura J, Carvalho E, Empadinhas N. Microbiota of chronic diabetic wounds: Ecology, impact, and potential for innovative treatment strategies. Front Microbiol. 2017;8(SEP):1-12.
Peters EJ, Lipsky BAJ, Aragón-Sánchez EJ, Boyko, Diggle M, J.M.Embil S, et al. Interventions in the management of infection in the foot in diabetes: a systematic review. Diabetes Metab Res Rev. 2016;32(1):145-53.
Lima AF, Costa LB, Silva JL Da, Maia MBS, Ximenes ECPA. Interventions for wound healing among diabetic patients infected with Staphylococcus aureus: a systematic review. Sao Paulo Med J. 2011;129(3):165-70.
Nube V, Frank G, White J, Stubbs S, Nannery S, Pfrunder L, et al. Hard-to-heal diabetes-related foot ulcers: current challenges and future prospects. 2016;133-46.
Anderson K, Hamm RL. Factors that impair wound healing. J Am Coll Clin Wound Spec [Internet]. 2012;4(4):84-91. Available from: http://dx.doi.org/10.1016/j.jccw.2014.03.001
Alexiadou K, Doupis J. Management of Diabetic Foot Ulcers. Diabetes Ther. 2012;3(4):1-15.
Rustenbach SJ, Augustin M, Herberger K, Spehr C, Heyer K, Protz K. Effectiveness of Advanced versus Conventional Wound Dressings on Healing of Chronic Wounds: Systematic Review and Meta-Analysis. Dermatology. 2013;226(2):172-84.
Tricco AC, Cogo E, Isaranuwatchai W, Khan PA, Sanmugalingham G, Antony J, et al. A systematic review of cost-effectiveness analyses of complex wound interventions reveals optimal treatments for specific wound types. BMC Med. 2015;13(1):1-16.
Kvitkina T, Narres M, Claessen H, Droste S, Morbach S, Kuss O, et al. Incidence of lower extremity amputation in the diabetic compared to the non-diabetic population: A systematic review protocol. Syst Rev [Internet]. 2015;4(1). Available from: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L605593026%5Cnhttp://dx.doi.org/10.1186/s13643-015-0064-9%5Cnhttp://sfx.library.uu.nl/utrecht?sid=EMBASE&issn=20464053&id=doi:10.1186%2Fs13643-015-0064-9&atitle=Incidence+of+lower+ex
Alqadri, Tambing Y, Latarang B. Morphological and anatomical characteristics of mangosteen (Garcinia mangostana L.). Agrotekbis. 2016;4(5):571-8.
Nainwal P, Bhagla.A, Nanda.D. Study on antioxidant potential and wound healing activity on the aqueous extract of fruits of Garcinia mangostana. IJPI's J Pharmacogn Herb Formul. 2014;1. Study on antioxidant potential and wound healing activity on the a. IJPI's J Pharmacogn Herb Formul. 2014;1.
Widowati W, Darsono L, Suherman J, Fauziah N, Maesaroh M, Erawijantari P putu. Anti-inflammatory effect of mangosteen (Garcinia mangostana l.) peel extract and its compounds in lps-induced raw264.7 cells. Nat Prod Sci. 2016;22(3):147-53.
Jindarat S. Xanthones from mangosteen (Garcinia mangostana): Multi-targeting pharmacological properties. J Med Assoc Thail. 2014;97(2):S196-201.
Taher M, Muhamad T, Syafiq F, Zakaria T, Susanti D, Zakaria ZA. Hypoglycaemic activity of ethanolic extract of Garcinia mangostana Linn. in normoglycaemic and streptozotocin- induced diabetic rats. BMC Complement Altern Med [Internet]. 2016;16(135):1-12. Available from: http://dx.doi.org/10.1186/s12906-016-1118-9
Nganlasom J, Suttitum T, Jirakulsomchok D, Puapairoj A. Effects of Centella Asiatica Linn. Leaves and Garcinia Mangostana Linn. hull on the Healing of Dermal Wounds in Diabetic Rats. Srinagarind Med J. 2008;23(4):402-7.
Cholilah, Ellistasari EY, Radiono S. Effects of 5%, 10%, 20% and 40% mangosteen (garcinia mangostana l.) peel extract cream on wound healing in vivo on mice skin [Internet]. Gadjah Mada University; 2017. Available from: http://etd.repository.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=109968&obyek_id=4
Aalaa M, Malazy OT, Sanjari M, Peimani M, Mohajeri-Tehrani MR. Nurses' role in diabetic foot prevention and care; a review. J Diabetes Metab Disord. 2012;11(1):1-6.
Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem [Internet]. 2017;34:540-60. Available from: http://dx.doi.org/10.1016/j.ultsonch.2016.06.035
Santos D, Vardanega R, De Almeida MA. Intensification of bioactive compounds extraction from medicinal plants using ultrasonic irradiation. Pharmacogn Rev [Internet]. 2014;8(16):88. Available from: http://www.phcogrev.com/text.asp?2014/8/16/88/134231
Alberti T, Coelho DS, Voytena A, Pitz H, Prá M De, Mazzarino L. Nanotechnology: A Promising Tool Towards Wound Healing. Pharmaceutical. 2017;23(January 2018):1.
Goyal R, Macri LK, Kaplan HM, Kohn J. Nanoparticles and nanofibers for topical drug delivery. J Control Release [Internet]. 2016 [cited 2019 Jun 27];240:77-92. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26518723
Singla R, Soni S, Patial V, Kulurkar PM, Kumari A, Mahesh S, et al. In vivo diabetic wound healing potential of nanobiocomposites containing bamboo cellulose nanocrystals impregnated with silver nanoparticles. Int J Biol Macromol [Internet]. 2017;105:45-55. Available from: http://dx.doi.org/10.1016/j.ijbiomac.2017.06.109
Patra JK, Das G, Fraceto LF, Vangelie E, Campos R, Rodriguez P, et al. Nano-based drug delivery systems: recent developments and future prospects. J Nanobiotechnology [Internet]. 2018;1-33. Available from: https://doi.org/10.1186/s12951-018-0392-8
Das S, Baker AB. Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing. Front Bioeng Biotechnol [Internet]. 2016;4(October):1–20. Available from: http://journal.frontiersin.org/article/10.3389/fbioe.2016.00082/full
Schneider M, Stracke F, Hansen S, Schaefer UF. Nanoparticles and their interactions with the dermal barrier. Dermatoendocrinol. 2010;1(4):197-206.
Grice JE, Zvyagin A V, Roberts M, Liu X. Penetration of Nanoparticles into Human Skin. Curr Pharm Des. 2013;19(March):6353.
Wani A, Sanghani C, Wani S. Formulation, Characterization, and in Vitro Evaluation of Novel Microemulsion-Based Spray for Topical Delivery of Isotretinoin. Asian J Pharm Clin Res. 2018;11(10):226.
Hasan AEZ, Nashrianto H, Juhaeni RN, Artika IM. Optimization of conditions for flavonoids extraction from Mangosteen (Garcinia mangostana L.). Der Pharm Lett. 2016;8(18):114-20.
