Antioxidant Capacity Test and Phenolic Test of Coriander Leaf Extract (Coriandrum sativum L.) with ABTS Method
Main Article Content
Coriander leaves, also known as cilantro, are part of the Coriandrum sativum L. plant that is widely used in cuisines around the world and has potential as a medicinal ingredient. It has a distinctive aroma and a fresh, slightly spicy, citrusy flavor and is often used as an ingredient in cooking. The plant can be recognized by its upright, hairless form and abundant branches. The leaves vary from thick at the base of the plant to slender and hairy at the top of the flowering stems. Coriander leaves have functions as health medicine, among others, as a remedy for relieving digestive, respiratory, and urinary tract problems. This study aims to evaluate the antioxidant and phenolic activities of the methanol extract of coriander leaves. Coriander leaves were extracted with methanol and tested for antioxidant activity using the DPPH method. This study used two main methods: in vitro and bioassay, to evaluate the samples. The in vitro method consisted of an antioxidant capacity test. The antioxidant capacity assay aims to measure the ability of the sample to neutralize free radicals. The total phenolic assay is used as a method to measure antioxidant capacity, by calculating the levels of phenolic compounds in the sample. The total phenolic content of coriander leaf extract was found to be 726.0 ?g/mL. DPPH antioxidant capacity test obtained 132.12 ?g/mL.
Bhat, S., Kaushal, P., Kaur, M., & Sharma, H. K. (2014). Coriander (Coriandrum sativum L.): Processing, nutritional and functional aspects. African Journal of Plant Science, 8(1), 25–33.
Ghina, M., Yuniarti, E., & Atifah, Y. (2023). Literature Review: Potential of Coriander (Coriandrum sativum L.) as a Source of Natural Antioxidants. Jurnal Biologi Tropis, 23(1), 166–172. https://doi.org/10.29303/jbt.v23i4b.5610
Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023). Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Archives of Toxicology, 97(10), 2499–2574. https://doi.org/10.1007/s00204-023-03562-9
Juan, C. A., Pérez de la Lastra, J. M., Plou, F. J., & Pérez-Lebeña, E. (2021). The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences, 22(9), 4642. https://doi.org/10.3390/ijms22094642
Kassahun, B. M. (2020). Unleashing the exploitation of coriander (Coriander sativum L.) for biological, industrial and pharmaceutical applications. Academic Research Journal of Agricultural Science and Research, 8(6), 552–564.
Krumova, K., & Cosa, G. (2016). Overview of reactive oxygen species.
Mahleyuddin, N. N., Moshawih, S., Ming, L. C., Zulkifly, H. H., Kifli, N., Loy, M. J., Sarker, Md. M. R., Al-Worafi, Y. M., Goh, B. H., Thuraisingam, S., & Goh, H. P. (2021). Coriandrum sativum L.: A Review on Ethnopharmacology, Phytochemistry, and Cardiovascular Benefits. Molecules, 27(1), 209. https://doi.org/10.3390/molecules27010209
Önder, A. (2018). Coriander and its phytoconstituents for the beneficial effects. Potential of Essential Oils, 165.
Ozougwu, J. C. (2016). The role of reactive oxygen species and antioxidants in oxidative stress. International Journal of Research, 1(8), 1–8.
Pisoschi, A. M., & Pop, A. (2015). The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry, 97, 55–74. https://doi.org/10.1016/j.ejmech.2015.04.040
Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., & Dhama, K. (2014). Oxidative Stress, Prooxidants, and Antioxidants: The Interplay. BioMed Research International, 2014, 1–19. https://doi.org/10.1155/2014/761264
Simpson, D. S. A., & Oliver, P. L. (2020). ROS Generation in Microglia: Understanding Oxidative Stress and Inflammation in Neurodegenerative Disease. Antioxidants, 9(8), 743. https://doi.org/10.3390/antiox9080743
Singh, A., Kukreti, R., Saso, L., & Kukreti, S. (2019). Oxidative Stress: A Key Modulator in Neurodegenerative Diseases. Molecules, 24(8), 1583. https://doi.org/10.3390/molecules24081583
Umeno, A., Biju, V., & Yoshida, Y. (2017). In vivo ROS production and use of oxidative stress-derived biomarkers to detect the onset of diseases such as Alzheimer’s disease, Parkinson’s disease, and diabetes. Free Radical Research, 51(4), 413–427. https://doi.org/10.1080/10715762.2017.1315114
Vasco, C., Ruales, J., & Kamal-Eldin, A. (2008). Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry, 111(4), 816–823. https://doi.org/10.1016/j.foodchem.2008.04.054