Phytochemical Test, Total Antioxidant Capacity and Toxicity of Lobi-Lobi Fruit Extract
(Flacourtia Inermis)
Main Article Content
This study aims to determine information on phytochemical tests, total antioxidant capacity and toxicity of lobi-lobi fruit extract (Flacourtia Inermis). As we know, the Lobi-lobi fruit itself is small and looks round, the ripe Lobi-lobi fruit is usually dark red in color and has a sour taste, but often it tastes astringent and has seeds. In Indonesia, the lobi-lobi fruit is usually used to make rujak, syrup, sweets, pickles and canned fruit. However, even though it has quite a lot of potential, it turns out that the fruits of lobbying are still not widely known by the Indonesian people. Apart from that, information about the contents of the lobi-lobi fruit is still not widely known to the Indonesian public. By using experimental research methods that are in vitro and bioassay. The in vitro test consists of a phytochemical test, antioxidant capacity test using a total phenolic test. Bioassay examination using Artemia Salina larvae using the BSLT method. Research results show that Lobi-lobi fruit extract contains alkaloids, cardioglycosides, glycosides, flavonoids, steroids, saponins, quinones, coumarins, anthocyanins, betacyanins, phenolics and terpenoids. The total phenolic content of Lobi-lobi fruit extract was 1492.5 ?g/mL. The DPPH antioxidant capacity test results were 104,223 ?g/mL. The ABTS antioxidant capacity test results obtained were 29.818?g/mL. The FRAP antioxidant capacity test results obtained were 104,223 ?g/mL. As well as the toxicity of Lobi-lbi fruit extract, the LC50 result was 158,489 ?g/mL.
Basuki, I., Adinugroho, W. C., Utomo, N. A., Syaugi, A., Tryanto, D. H., Krisnawati, H., Cook-Patton, S. C., & Novita, N. (2022). Reforestation Opportunities in Indonesia: Mitigating Climate Change and Achieving Sustainable Development Goals. Forests, 13(3), 447. https://doi.org/10.3390/f13030447
Can-Cauich, C. A., Sauri-Duch, E., Betancur-Ancona, D., Chel-Guerrero, L., González-Aguilar, G. A., Cuevas-Glory, L. F., Pérez-Pacheco, E., & Moo-Huchin, V. M. (2017). Tropical fruit peel powders as functional ingredients: Evaluation of their bioactive compounds and antioxidant activity. Journal of Functional Foods, 37, 501–506. https://doi.org/10.1016/j.jff.2017.08.028
Dahono, D., & Zurriyati, Y. (2018). KERAGAMAN SUMBERDAYA GENETIK TANAMAN BUAH-BUAHAN EKSOTIK DI KABUPATEN BINTAN PROVINSI KEPULAUAN RIAU. Buletin Plasma Nutfah, 22(1), 11. https://doi.org/10.21082/blpn.v22n1.2016.p11-20
Indrayanto, G., Putra, G. S., & Suhud, F. (2021). Validation of in-vitro bioassay methods: Application in herbal drug research (pp. 273–307). https://doi.org/10.1016/bs.podrm.2020.07.005
Jia, A., Escher, B. I., Leusch, F. D. L., Tang, J. Y. M., Prochazka, E., Dong, B., Snyder, E. M., & Snyder, S. A. (2015). In vitro bioassays to evaluate complex chemical mixtures in recycled water. Water Research, 80, 1–11. https://doi.org/10.1016/j.watres.2015.05.020
Kumoro, A. C., Alhanif, M., & Wardhani, D. H. (2020). A Critical Review on Tropical Fruits Seeds as Prospective Sources of Nutritional and Bioactive Compounds for Functional Foods Development: A Case of Indonesian Exotic Fruits. International Journal of Food Science, 2020, 1–15. https://doi.org/10.1155/2020/4051475
Moo-Huchin, V. M., Moo-Huchin, M. I., Estrada-León, R. J., Cuevas-Glory, L., Estrada-Mota, I. A., Ortiz-Vázquez, E., Betancur-Ancona, D., & Sauri-Duch, E. (2015). Antioxidant compounds, antioxidant activity and phenolic content in peel from three tropical fruits from Yucatan, Mexico. Food Chemistry, 166, 17–22. https://doi.org/10.1016/j.foodchem.2014.05.127
Pereira-Netto, A. B. (2018). Tropical Fruits as Natural, Exceptionally Rich, Sources of Bioactive Compounds. International Journal of Fruit Science, 18(3), 231–242. https://doi.org/10.1080/15538362.2018.1444532
Rahman, S. (2018). Membangun pertanian dan pangan untuk mewujudkan kedaulatan pangan. Deepublish.
Salmiyah, S., & Bahruddin, A. (2018). Fitokimia dan antioksidan pada buah tome-tome (Flacourtia inermis). Hospital Majapahit (Jurnal Ilmiah Kesehatan Politeknik Kesehatan Majapahit Mojokerto), 10(1).
Sarkar, T., Salauddin, M., Roy, A., Sharma, N., Sharma, A., Yadav, S., Jha, V., Rebezov, M., Khayrullin, M., Thiruvengadam, M., Chung, I.-M., Shariati, M. A., & Simal-Gandara, J. (2023). Minor tropical fruits as a potential source of bioactive and functional foods. Critical Reviews in Food Science and Nutrition, 63(23), 6491–6535. https://doi.org/10.1080/10408398.2022.2033953
SWARA, P., & Risa, I. (2012). Hanya Ada di Indonesia: 1100+ Keajaiban dan Prestasi yang Mendunia. Puspa Swara.
Umam, M. N., Poerwanto, R., & Matra, D. D. (2023). Morphological and phytochemical characterization of Lobi-lobi fruit (Flacourtia inermis) at each maturity stage. 050007. https://doi.org/10.1063/5.0135756
Yasin, F. M., Zam, Z. Z., & Rakhman, K. A. (2022). ANALYSIS OF ANTIOXIDANT CONTENT OF ANTHOCYANIN IN THE LOBI-LOBI FRUIT (Flacourtian inermis) AND JAMBLANG FRUIT (Syzygium cumini L Skeel) USING THE DPPH METHOD WITH SPECTROPHOTOMETRY. Jurnal Biosains Pascasarjana, 24(1).
Yu, X., Zhang, Y., Yang, M., Guo, J., Xu, W., Gao, J., Li, Y., & Tao, L. (2016). Cytotoxic effects of tebufenozide in vitro bioassays. Ecotoxicology and Environmental Safety, 129, 180–188. https://doi.org/10.1016/j.ecoenv.2016.03.025