Good human resources are one of the crucial factors in health care institutions. Improving employee performance and achievement will have an impact on improving company performance. This study aims to determine the extent to which radiology staff performance is influenced by organizational culture, competence, and motivation and implement performance improvement using performance appraisals and potential estimates. The object of this research is the three branches of the XYZ hospital group. The sample in this study used saturated sampling, with the entire population being the sample. Statistical tests use SEM PLS. Based on the analysis results, it is proven that organizational culture and competence have a positive and significant effect on the performance of radiology staff. Organizational culture and competence have a positive and significant effect on the work motivation of radiology staff. Motivation can mediate the indirect influence of organizational culture and competence on the performance of radiology staff. Implementation of performance improvement models using performance assessment and potential estimates. All hypotheses in this research are proven and acceptable. Organizational culture and competence directly and indirectly affect the performance and motivation of radiology staff.