Dermal Acute Toxicity Test
Main Article Content
The aim of the dermal acute toxicity test is to identify the intrinsic toxicity of a product by assessing its hazard potential following acute skin exposure. This test also provides preliminary data that can be used to determine the appropriate dosage level and guide the design of subsequent toxicity studies, including determining the LD50 value and information about skin absorption. In this study, female rats (Rattus Nervegicus) are used as test subjects. The test material is prepared by diluting 15 ml of the sample to a final concentration of 994.9 mg/mL. The rats are exposed to the material for 24 hours. Post-exposure, microanatomical observations, and surgery in the abdominal and thoracic areas revealed no abnormalities.
Keywords:
In Vivo, Toxicity Test, Acute Dermal
Berggren, E., White, A., Ouedraogo, G., Paini, A., Richarz, A.-N., Bois, F. Y., … Mahony, C. (2017). Ab initio chemical safety assessment: A workflow based on exposure considerations and non-animal methods. Computational Toxicology, 4, 31–44. Retrieved from https://doi.org/10.1016/j.comtox.2017.10.001
De Santis, S., Carozzi, F. G., de Felice, G., & Poggi, C. (2017). Test methods for Textile Reinforced Mortar systems. Composites Part B: Engineering, 127, 121–132. Retrieved from https://doi.org/10.1016/j.compositesb.2017.03.016
Deasy Andani, G. A. S., Lesmana, R., & Pratiwi, Y. S. (2022). The Role of Herbs Supplementation in Modulating Injury-induced Inflammatory Response in Skeletal Muscle: A Systematic Review. The Natural Products Journal, 12(7), 62–72.
Demirci, T., & Sahin, E. (2019). The effect of chronic stress and obesity on sperm quality and testis histology in male rats; a morphometric and immunohistochemical study.
Development, O. for E. C. and. (2015). Test No. 404: acute dermal irritation/corrosion. OECD Publishing.
Erkekoglu, P., & Kocer-Gumusel, B. (2018). Toxicity assessment of nanopharmaceuticals. In Inorganic Frameworks as Smart Nanomedicines (pp. 565–603). Elsevier. Retrieved from https://doi.org/10.1016/B978-0-12-813661-4.00013-4
Guérin, G., Christophe, C., Philippe, A., Murray, A. S., Thomsen, K. J., Tribolo, C., … Lahaye, C. (2017). Absorbed dose, equivalent dose, measured dose rates, and implications for OSL age estimates: Introducing the Average Dose Model. Quaternary Geochronology, 41, 163–173. Retrieved from https://doi.org/10.1016/j.quageo.2017.04.002
Hamm, J., Sullivan, K., Clippinger, A. J., Strickland, J., Bell, S., Bhhatarai, B., … Allen, D. (2017). Alternative approaches for identifying acute systemic toxicity: Moving from research to regulatory testing. Toxicology in Vitro, 41, 245–259. Retrieved from https://doi.org/10.1016/j.tiv.2017.01.004
Hernández-López, V., Reyes, R., García-Álvarez, N., Real, F., Díaz-Marrero, A. R., & Fernández, J. J. (2024). Changes at small intestine induced by food-fish contaminated with ciguatoxins. Ecotoxicology and Environmental Safety, 282, 116741. Retrieved from https://doi.org/10.1016/j.ecoenv.2024.116741
McGonigle, P. (2014). Animal models of CNS disorders. Biochemical Pharmacology, 87(1), 140–149. Retrieved from https://doi.org/10.1016/j.bcp.2013.06.016
Strickland, J., Clippinger, A. J., Brown, J., Allen, D., Jacobs, A., Matheson, J., … Casey, W. (2018). Status of acute systemic toxicity testing requirements and data uses by U.S. regulatory agencies. Regulatory Toxicology and Pharmacology, 94, 183–196. Retrieved from https://doi.org/10.1016/j.yrtph.2018.01.022
Sun, K., Song, Y., He, F., Jing, M., Tang, J., & Liu, R. (2021). A review of human and animals exposure to polycyclic aromatic hydrocarbons: Health risk and adverse effects, photo-induced toxicity and regulating effect of microplastics. Science of The Total Environment, 773, 145403. Retrieved from https://doi.org/10.1016/j.scitotenv.2021.145403
Tollefsen, K. E., Scholz, S., Cronin, M. T., Edwards, S. W., de Knecht, J., Crofton, K., … Patlewicz, G. (2014). Applying Adverse Outcome Pathways (AOPs) to support Integrated Approaches to Testing and Assessment (IATA). Regulatory Toxicology and Pharmacology, 70(3), 629–640. Retrieved from https://doi.org/10.1016/j.yrtph.2014.09.009
Yun, J.-W., Kwon, E., Kim, Y.-S., Kim, S.-H., You, J.-R., Kim, H.-C., … Jang, J.-J. (2018). Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats. BMC Complementary and Alternative Medicine, 18, 1–12.
Zwickl, C. M., Graham, J. C., Jolly, R. A., Bassan, A., Ahlberg, E., Amberg, A., … Myatt, G. J. (2022). Principles and procedures for assessment of acute toxicity incorporating in silico methods. Computational Toxicology, 24, 100237. Retrieved from https://doi.org/10.1016/j.comtox.2022.100237
De Santis, S., Carozzi, F. G., de Felice, G., & Poggi, C. (2017). Test methods for Textile Reinforced Mortar systems. Composites Part B: Engineering, 127, 121–132. Retrieved from https://doi.org/10.1016/j.compositesb.2017.03.016
Deasy Andani, G. A. S., Lesmana, R., & Pratiwi, Y. S. (2022). The Role of Herbs Supplementation in Modulating Injury-induced Inflammatory Response in Skeletal Muscle: A Systematic Review. The Natural Products Journal, 12(7), 62–72.
Demirci, T., & Sahin, E. (2019). The effect of chronic stress and obesity on sperm quality and testis histology in male rats; a morphometric and immunohistochemical study.
Development, O. for E. C. and. (2015). Test No. 404: acute dermal irritation/corrosion. OECD Publishing.
Erkekoglu, P., & Kocer-Gumusel, B. (2018). Toxicity assessment of nanopharmaceuticals. In Inorganic Frameworks as Smart Nanomedicines (pp. 565–603). Elsevier. Retrieved from https://doi.org/10.1016/B978-0-12-813661-4.00013-4
Guérin, G., Christophe, C., Philippe, A., Murray, A. S., Thomsen, K. J., Tribolo, C., … Lahaye, C. (2017). Absorbed dose, equivalent dose, measured dose rates, and implications for OSL age estimates: Introducing the Average Dose Model. Quaternary Geochronology, 41, 163–173. Retrieved from https://doi.org/10.1016/j.quageo.2017.04.002
Hamm, J., Sullivan, K., Clippinger, A. J., Strickland, J., Bell, S., Bhhatarai, B., … Allen, D. (2017). Alternative approaches for identifying acute systemic toxicity: Moving from research to regulatory testing. Toxicology in Vitro, 41, 245–259. Retrieved from https://doi.org/10.1016/j.tiv.2017.01.004
Hernández-López, V., Reyes, R., García-Álvarez, N., Real, F., Díaz-Marrero, A. R., & Fernández, J. J. (2024). Changes at small intestine induced by food-fish contaminated with ciguatoxins. Ecotoxicology and Environmental Safety, 282, 116741. Retrieved from https://doi.org/10.1016/j.ecoenv.2024.116741
McGonigle, P. (2014). Animal models of CNS disorders. Biochemical Pharmacology, 87(1), 140–149. Retrieved from https://doi.org/10.1016/j.bcp.2013.06.016
Strickland, J., Clippinger, A. J., Brown, J., Allen, D., Jacobs, A., Matheson, J., … Casey, W. (2018). Status of acute systemic toxicity testing requirements and data uses by U.S. regulatory agencies. Regulatory Toxicology and Pharmacology, 94, 183–196. Retrieved from https://doi.org/10.1016/j.yrtph.2018.01.022
Sun, K., Song, Y., He, F., Jing, M., Tang, J., & Liu, R. (2021). A review of human and animals exposure to polycyclic aromatic hydrocarbons: Health risk and adverse effects, photo-induced toxicity and regulating effect of microplastics. Science of The Total Environment, 773, 145403. Retrieved from https://doi.org/10.1016/j.scitotenv.2021.145403
Tollefsen, K. E., Scholz, S., Cronin, M. T., Edwards, S. W., de Knecht, J., Crofton, K., … Patlewicz, G. (2014). Applying Adverse Outcome Pathways (AOPs) to support Integrated Approaches to Testing and Assessment (IATA). Regulatory Toxicology and Pharmacology, 70(3), 629–640. Retrieved from https://doi.org/10.1016/j.yrtph.2014.09.009
Yun, J.-W., Kwon, E., Kim, Y.-S., Kim, S.-H., You, J.-R., Kim, H.-C., … Jang, J.-J. (2018). Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats. BMC Complementary and Alternative Medicine, 18, 1–12.
Zwickl, C. M., Graham, J. C., Jolly, R. A., Bassan, A., Ahlberg, E., Amberg, A., … Myatt, G. J. (2022). Principles and procedures for assessment of acute toxicity incorporating in silico methods. Computational Toxicology, 24, 100237. Retrieved from https://doi.org/10.1016/j.comtox.2022.100237
